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Optimization of a peptide-based protocol employing IL-7 for in vitro restimulation of human cytotoxic T lymphocyte precursors.

Abstract:

A variety of different methods for the in vitro restimulation of human cytotoxic T lymphocyte (CTL) precursors (CTLp) are in use. Our aim was to enhance the detection of circulating human CTLp in peripheral blood. We have developed a standardized and highly efficient method for restimulating CTLp. Synthetic peptides were used to restimulate cognate CTLp from peripheral blood mononuclear cells (PBMC), and effector CTL capable of lysing peptide-pulsed and virus infected targets were generated. ...

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Publication status:
Published

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Authors


Lalvani, A More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Patham, AA More by this author
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Journal:
Journal of immunological methods
Volume:
210
Issue:
1
Pages:
65-77
Publication date:
1997-12-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:98f0255b-7a97-44d9-85f8-df933471a054
Source identifiers:
36155
Local pid:
pubs:36155

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