Journal article
Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
- Abstract:
- Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.8MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-017-19063-6
Authors
+ Medical Research Council
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- Funding agency for:
- Reyes-Sandoval, A
- Grant:
- MR/N019008/1
+ Oxford Martin School
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- Funding agency for:
- Reyes-Sandoval, A
- Grant:
- MR/N019008/1
+ Jenner investigator
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- Funding agency for:
- Reyes-Sandoval, A
- Grant:
- MR/N019008/1
+ Wellcome Trust
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- Funding agency for:
- Andolina, C
- Nosten, F
- Grant:
- 089179
- 089179
+ FAPESP
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- Funding agency for:
- Gimenez, A
- Lima, L
- Grant:
- 2014/18102-7
- 2009/15099-7
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Volume:
- 8
- Pages:
- 1-14
- Publication date:
- 2018-01-18
- Acceptance date:
- 2017-12-21
- DOI:
- EISSN:
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2045-2322
- ISSN:
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2045-2322
- Pmid:
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29348479
- Language:
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English
- Keywords:
- Pubs id:
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pubs:821284
- UUID:
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uuid:973e7216-7707-4c34-ab47-27d38edd9ccb
- Local pid:
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pubs:821284
- Source identifiers:
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821284
- Deposit date:
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2018-01-30
Terms of use
- Copyright holder:
- © Mendes de Camargo, et al 2018
- Copyright date:
- 2018
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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