A statistical comparison of silver and SYPRO Ruby staining for proteomic analysis.
Silver staining has been the method most commonly employed for high sensitivity staining of proteins following two-dimensional gel electrophoresis. Whilst this method offers detection in the nanogram range it does have major drawbacks including a lack of linearity, nonstoichiometric staining of proteins, a lack of compatibility with the microchemical preparation of proteins for identification by mass spectrometric techniques, and a highly subjective assessment of the staining endpoint. SYPRO ...Expand abstract
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- Wiley-VCH Verlag GmbH and Co.
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