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A statistical comparison of silver and SYPRO Ruby staining for proteomic analysis.

Abstract:

Silver staining has been the method most commonly employed for high sensitivity staining of proteins following two-dimensional gel electrophoresis. Whilst this method offers detection in the nanogram range it does have major drawbacks including a lack of linearity, nonstoichiometric staining of proteins, a lack of compatibility with the microchemical preparation of proteins for identification by mass spectrometric techniques, and a highly subjective assessment of the staining endpoint. SYPRO ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/elps.200405947

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
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Publisher:
Wiley-VCH Verlag Publisher's website
Journal:
Electrophoresis Journal website
Volume:
25
Issue:
17
Pages:
3048-3054
Publication date:
2004-09-05
DOI:
EISSN:
1522-2683
ISSN:
0173-0835
URN:
uuid:96c4f910-786b-49da-ade9-d2a004b27ea8
Source identifiers:
264657
Local pid:
pubs:264657

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