Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis.

Journal article

A collaborative project between two Structural Proteomics In Europe (SPINE) partner laboratories, York and Oxford, aimed at high-throughput (HTP) structure determination of proteins from Bacillus anthracis, the aetiological agent of anthrax and a biomedically important target, is described. Based upon a target-selection strategy combining ;low-hanging fruit' and more challenging targets, this work has contributed to the body of knowledge of B. anthracis, established and developed HTP cloning ...

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Authors: Au, K; Berrow, N; Blagova, E; Boucher, I; Boyle, M

• Publication status: Published
• Journal: Acta crystallographica. Section D, Biological crystallography
• Volume: 62
• Issue: Pt 10
• Pages: 1267-1275
• Publication date: 2006-10-01
• DOI: 10.1107/s0907444906033555
• EISSN: 1399-0047
• ISSN: 0907-4449
• Language: English
• Keywords: Bacterial Proteins; Spores, Bacterial; Genetic Vectors; Computational Biology; Reverse Transcriptase Polymerase Chain Reaction; Magnetic Resonance Spectroscopy; Bacillus cereus; Crystallization; RNA, Transfer; Proteomics; DNA, Bacterial; Sulfurtransferases; Bacillus anthracis; Escherichia coli; Cloning, Molecular; Crystallography, X-Ray; Robotics