Journal article
Confocal imaging of glutathione redox potential in living plant cells.
- Abstract:
-
Reduction-oxidation-sensitive green fluorescent protein (roGFP1 and roGFP2) were expressed in different sub-cellular compartments of Arabidopsis and tobacco leaves to empirically determine their performance as ratiometric redox sensors for confocal imaging in planta. A lower redox-dependent change in fluorescence in combination with reduced excitation efficiency at 488 nm resulted in a significantly lower dynamic range of roGFP1 than for roGFP2. Nevertheless, when targeted to the cytosol and ...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of microscopy More from this journal
- Volume:
- 231
- Issue:
- 2
- Pages:
- 299-316
- Publication date:
- 2008-08-01
- DOI:
- EISSN:
-
1365-2818
- ISSN:
-
0022-2720
Item Description
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:34376
- UUID:
-
uuid:96616482-19c0-46e1-9c4a-2868904e9ab8
- Local pid:
-
pubs:34376
- Source identifiers:
-
34376
- Deposit date:
-
2012-12-19
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- Copyright date:
- 2008
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