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Confocal imaging of glutathione redox potential in living plant cells.

Abstract:

Reduction-oxidation-sensitive green fluorescent protein (roGFP1 and roGFP2) were expressed in different sub-cellular compartments of Arabidopsis and tobacco leaves to empirically determine their performance as ratiometric redox sensors for confocal imaging in planta. A lower redox-dependent change in fluorescence in combination with reduced excitation efficiency at 488 nm resulted in a significantly lower dynamic range of roGFP1 than for roGFP2. Nevertheless, when targeted to the cytosol and ...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
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Journal:
Journal of microscopy
Volume:
231
Issue:
2
Pages:
299-316
Publication date:
2008-08-05
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
URN:
uuid:96616482-19c0-46e1-9c4a-2868904e9ab8
Source identifiers:
34376
Local pid:
pubs:34376

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