Targeted analysis of protein termini.

Journal article

We describe a targeted analysis of protein isoforms by selective enrichment and identification of in vivo acetylated protein N-termini and protein C-termini. Our method allows the characterization of these protein termini regardless of their annotation in protein databases and requires no chemical derivatization. Using an iterative database search strategy that takes account of the enrichment protocol, 263 IPI annotated and 87 unpredicted acetylated N-termini were identified in the crude memb...

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Authors: Dormeyer, W; Mohammed, S; Breukelen, B; Krijgsveld, J; Heck, A

• Publication status: Published
• Journal: Journal of proteome research
• Volume: 6
• Issue: 12
• Pages: 4634-4645
• Publication date: 2007-12-01
• DOI: 10.1021/pr070375k
• EISSN: 1535-3907
• ISSN: 1535-3893
• Language: English
• Keywords: Humans; Amino Acid Sequence; Acetylation; Sequence Analysis, Protein; Chemical Fractionation; Peptide Fragments; Molecular Sequence Data; Protein Isoforms; Proteins; Databases, Protein; Cell Line, Tumor