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Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle.

Abstract:

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CDelta5) and diffraction data were collected to 1.9 A resolution. The crystals belong to space group C2, with unit-cell parameters a = 183.4, b = 28.1, c = 27.8 A, beta = 96.5 degrees. An anomalous difference Patterson map calculated with the data from the SeMet-lab...

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Publication status:
Published

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Publisher copy:
10.1107/s1744309106006555

Authors


Cordes, FS More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Johnson, S More by this author
Kenjale, R More by this author
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Journal:
Acta crystallographica. Section F, Structural biology and crystallization communications
Volume:
62
Issue:
Pt 3
Pages:
302-305
Publication date:
2006-03-05
DOI:
EISSN:
1744-3091
ISSN:
1744-3091
URN:
uuid:95f91ef9-c777-4df0-ab95-9b05df82ac73
Source identifiers:
15212
Local pid:
pubs:15212

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