Chapter 21 - How to detect and purify tenascin-C

Journal article

The extracellular matrix molecule tenascin-C (TNC) was discovered over 30 years ago, and its tightly regulated pattern of expression since sparked keen interest in the scientific community. In adult tissues, TNC expression is restricted to specific niches and areas of active remodeling or high mechanical strain. However, while most healthy tissues contain little TNC, its transient expression upon cellular stress or tissue injury helps to mediate repair and restore homeostasis. Persistent expr...

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Authors: Giblin, S; Murdamoothoo, D; Deligne, C; Schwenzer, A; Orend, G

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Elsevier
• Journal: Methods in Cell Biology
• Volume: 143
• Issue: 2018
• Pages: 371-400
• Publication date: 2017-11-26
• DOI: 10.1016/bs.mcb.2017.08.022
• ISSN: 0091-679X
• Language: English
• Keywords: Immunohistochemistry; ELISA; Immunofluorescence; Splicing; Purification; qPCR; Cell-derived matrix; shRNA-mediated knockdown; SBTMR; Tenascin-C tissue expression; Western blot