Measurement of specimen-induced aberrations of biological samples using phase stepping interferometry.

Journal article

Confocal or multiphoton microscopes, which deliver optical sections and three-dimensional (3D) images of thick specimens, are widely used in biology. These techniques, however, are sensitive to aberrations that may originate from the refractive index structure of the specimen itself. The aberrations cause reduced signal intensity and the 3D resolution of the instrument is compromised. It has been suggested to correct for aberrations in confocal microscopes using adaptive optics. In order to d...

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Authors: Schwertner, M; Booth, M; Neil, M; Wilson, T

• Publication status: Published
• Journal: Journal of microscopy
• Volume: 213
• Issue: Pt 1
• Pages: 11-19
• Publication date: 2004-01-01
• DOI: 10.1111/j.1365-2818.2004.01267.x
• EISSN: 1365-2818
• ISSN: 0022-2720
• Language: English
• Keywords: Animals; Caenorhabditis elegans; Female; Blastocyst; Oocytes; Mice; Refractometry; Interferometry; Microscopy, Confocal; Photons; Microscopy; Optics and Photonics