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Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion.

Abstract:

We have isolated artificial ligands or aptamers for infectious prions in order to investigate conformational aspects of prion pathogenesis. The aptamers are 2'-fluoro-modified RNA produced by in vitro selection from a large, randomized library. One of these ligands (aptamer SAF-93) had more than 10-fold higher affinity for PrPSc than for recombinant PrPC and inhibited the accumulation of PrPres in near physiological cell-free conversion assay. To understand the molecular basis of these proper...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m305297200

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Journal:
The Journal of biological chemistry
Volume:
278
Issue:
41
Pages:
39697-39705
Publication date:
2003-10-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:944b81ae-f9c2-459a-a5ab-c8fdc354096d
Source identifiers:
3771
Local pid:
pubs:3771

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