OBJECTIVE: To evaluate two contrasting methods of in vivo gene transfer into testicular cells using electroporation, with regard to efficiency of transfer and damage to the testes. DESIGN: Controlled animal study. SETTING: Research laboratory at a university medical school. ANIMAL(S): 8-10-week-old male mice. INTERVENTION(S): The reporter construct pCAGGS-LacZ consisting of a cytomegalovirus enhancer/chicken beta-actin promoter attached to the LacZ gene was introduced into the testes in vivo...Expand abstract
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Comparison of two methods of in vivo gene transfer by electroporation.
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