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Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template.

Abstract:

Next generation sequencing methods that can be applied to both the resequencing of whole genomes and to the selective resequencing of specific parts of genomes are needed. We describe (i) a massively scalable biochemistry, Cyclical Ligation and Cleavage (CycLiC) for contiguous base sequencing and (ii) apply it directly to a template captured on a microarray. CycLiC uses four color-coded DNA/RNA chimeric oligonucleotide libraries (OL) to extend a primer, a base at a time, along a template. The...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1093/nar/gkn906

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Scozzafava, G More by this author
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Funding agency for:
Mir, KU
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Funding agency for:
Mir, KU
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic acids research Journal website
Volume:
37
Issue:
1
Pages:
e5
Publication date:
2009-01-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:94261dbb-3750-4f73-9d4a-9f9594cb7d64
Source identifiers:
34487
Local pid:
pubs:34487

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