Nanoscopy of living brain slices with low light levels.

Journal article

Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution fluorescence microscopy called RESOLFT enables comparatively fast and continuous imaging of sensitive, nanosized features in living brain tissue. Using low-intensity illumination to switch photochromic fluorescent proteins reversibly between a fluorescent and a nonfluorescent state, ...

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Authors: Testa, I; Urban, N; Jakobs, S; Eggeling, C; Willig, K

• Journal: Neuron
• Volume: 75
• Issue: 6
• Pages: 992-1000
• Publication date: 2012-09-01
• DOI: 10.1016/j.neuron.2012.07.028
• EISSN: 1097-4199
• ISSN: 0896-6273
• Language: English
• Keywords: Animals; Microscopy, Confocal; Animals, Newborn; Mice; Mice, Inbred C57BL; Time Factors; Light; Patch-Clamp Techniques; Neurons; Nanotechnology; Dendritic Spines; Organ Culture Techniques; Hippocampus; Transfection; Image Processing, Computer-Assisted; Electric Stimulation; Long-Term Potentiation; Luminescent Proteins