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The antibody binding site. Labelling of a specific antibody against the photo-precursor of an aryl nitrene.

Abstract:

The isolation of specific rabbit antibodies for the haptenic group 4-azido-2-nitrophenyl, is described. These antibodies bind 1.8-2.0mol of hapten [in-(4-azido-2-nitrophenyl)-l-lysine]/mol with an association constant of nearly 10(7)m(-1) at 4 degrees C. On photolysis of the antibody-hapten complex, resulting in the formation of an aryl nitrene at the binding site, hapten was covalently bound to the antibody, and the antibody binding site was blocked. The ratio of labelling of heavy- and ligh...

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Publication status:
Published

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Journal:
The Biochemical journal
Volume:
128
Issue:
3
Pages:
499-508
Publication date:
1972-07-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:93baa904-e5c3-4344-a867-9ce2bc027f29
Source identifiers:
41560
Local pid:
pubs:41560

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