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Sensing DNA opening in transcription using quenchable Forster resonance energy transfer.

Abstract:

Many biological processes, such as gene transcription and replication, involve opening and closing of short regions of double-stranded DNA (dsDNA). Few techniques, however, can study these processes in real time or at the single-molecule level. Here, we present a Förster resonance energy transfer (FRET) assay that monitors the state of DNA (double- vs single-stranded) at a specific region within a DNA fragment, at both the ensemble level and the single-molecule level. The assay utilizes two ...

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Publication status:
Published

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Publisher copy:
10.1021/bi101184g

Authors


Santoso, Y More by this author
Tomescu, AI More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
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Journal:
Biochemistry
Volume:
49
Issue:
43
Pages:
9171-9180
Publication date:
2010-11-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:93b10a2c-ec7c-4582-9e08-859dbd7ccee0
Source identifiers:
71164
Local pid:
pubs:71164

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