Global gene expression analysis of human erythroid progenitors.

Journal article

Understanding the pattern of gene expression during erythropoiesis is crucial for a synthesis of erythroid developmental biology. Here, we isolated 4 distinct populations at successive erythropoietin-dependent stages of erythropoiesis, including the terminal, pyknotic stage. The transcriptome was determined using Affymetrix arrays. First, we demonstrated the importance of using defined cell populations to identify lineage and temporally specific patterns of gene expression. Cells sorted by su...

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Authors: Merryweather-Clarke, A; Atzberger, A; Soneji, S; Gray, N; Clark, K

• Publication status: Published
• Journal: Blood
• Volume: 117
• Issue: 13
• Pages: e96-108
• Publication date: 2011-03-01
• DOI: 10.1182/blood-2010-07-290825
• EISSN: 1528-0020
• ISSN: 0006-4971
• Language: English
• Keywords: Gene Expression Profiling; Erythroid Precursor Cells; Polymerase Chain Reaction; Cluster Analysis; Microarray Analysis; Cell Differentiation; Cells, Cultured; Gene Expression Regulation, Developmental; Humans; Erythropoiesis; Erythroblasts; Flow Cytometry