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Computational analysis of the number, area and density of gamma-H2AX foci in breast cancer cells exposed to (111)In-DTPA-hEGF or gamma-rays using Image-J software.

Abstract:

PURPOSE: To develop a simple method for the quantification of gamma-H2AX focus number, density and size. METHODS: MDA-MB-468 human breast cancer cells were treated overnight with (111)In-diethylenetriaminepentaacetic acid human epidermal growth factor ((111)In-DTPA-hEGF, 0-142 kBq/pmol) or exposed to gamma-radiation to induce DNA double strand breaks (DSB). DNA DSB formation was evaluated by detection of phosphorylated histone H2AX on serine 139 (gamma-H2AX) using immunofluorescence. Confocal...

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Publication status:
Published

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Publisher copy:
10.1080/09553000902748757

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Oncology
Role:
Author
Journal:
International journal of radiation biology More from this journal
Volume:
85
Issue:
3
Pages:
262-271
Publication date:
2009-03-01
DOI:
EISSN:
1362-3095
ISSN:
0955-3002
Language:
English
Keywords:
Pubs id:
pubs:131012
UUID:
uuid:9223495a-f031-4b23-a7e0-8238a0789055
Local pid:
pubs:131012
Source identifiers:
131012
Deposit date:
2012-12-19

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