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Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution

Abstract:

Bisulfite sequencing has been the gold standard for mapping DNA modifications including 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) for decades1,2,3,4. However, this harsh chemical treatment degrades the majority of the DNA and generates sequencing libraries with low complexity2,5,6. Here, we present a bisulfite-free and base-level-resolution sequencing method, TET-assisted pyridine borane sequencing (TAPS), for detection of 5mC and 5hmC. TAPS combines ten-eleven translocation (...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/s41587-019-0041-2

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Oxford Ludwig Institute
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Oxford Ludwig Institute
Oxford college:
Kellogg College
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Oxford Ludwig Institute
Oxford college:
Kellogg College
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Oxford Ludwig Institute
Oxford college:
St Cross College
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Oxford Ludwig Institute
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Biotechnology Journal website
Volume:
37
Pages:
424–429
Publication date:
2019-02-25
Acceptance date:
2019-01-22
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
Pubs id:
pubs:965542
URN:
uri:91d3f09f-9c55-497b-9dc2-103420db9343
UUID:
uuid:91d3f09f-9c55-497b-9dc2-103420db9343
Local pid:
pubs:965542

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