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Improved transcriptional activators and their use in mis-expression traps in Arabidopsis.

Abstract:

The synthetic transcription factor LhG4 has been used in numerous mis-expression studies in plants. We show that the sequence encoding the LhG4 activation domain, derived from Saccharomyces cerevisiae GAL4, contains several cryptic polyadenylation signals in Arabidopsis. The GAL4-derived sequence was modified according to preferred Arabidopsis codon usage, generating LhG4AtO which was faithfully transcribed in Arabidopsis plants. In protoplasts, LhG4AtO achieved maximum transactivation of the...

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Publication status:
Published

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Journal:
Plant journal : for cell and molecular biology More from this journal
Volume:
43
Issue:
5
Pages:
769-788
Publication date:
2005-09-01
DOI:
EISSN:
1365-313X
ISSN:
0960-7412

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