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Journal article

The proteomes of transcription factories containing RNA polymerases I, II or III.

Abstract:

Human nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammalian cells. After isolation, we analyzed their protein content by mass spectrometry. Each complex represents part of the core of a transcription factory. For example, the RNA polymerase II complex co...

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Publication status:
Published

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Publisher copy:
10.1038/nmeth.1705

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Nature methods More from this journal
Volume:
8
Issue:
11
Pages:
963-968
Publication date:
2011-11-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Language:
English
Keywords:
Pubs id:
pubs:180560
UUID:
uuid:912d0f6b-7885-4305-abb7-4447e86c9fc7
Local pid:
pubs:180560
Source identifiers:
180560
Deposit date:
2012-12-19

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