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Parallel reverse genetic screening in mutant human cells using transcriptomics

Abstract:

Reverse genetic screens have driven gene annotation and target discovery in model organisms. However, many disease‐relevant genotypes and phenotypes cannot be studied in lower organisms. It is therefore essential to overcome technical hurdles associated with large‐scale reverse genetics in human cells. Here, we establish a reverse genetic approach based on highly robust and sensitive multiplexed RNA sequencing of mutant human cells. We conduct 10 parallel screens using a collection of enginee...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.15252/msb.20166890

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Department:
Oxford, MSD, NDM, Oxford Ludwig Institute
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Department:
Oxford, MSD, NDM, Oxford Ludwig Institute
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Author
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Department:
Oxford, MSD, NDM, Target Discovery Institute
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Author
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Publisher:
EMBO Press Publisher's website
Journal:
Molecular Systems Biology Journal website
Volume:
12
Pages:
Article: 879
Publication date:
2016-08-01
Acceptance date:
2016-07-07
DOI:
ISSN:
1744-4292
Pubs id:
pubs:637450
URN:
uri:90784e8b-5a54-43ce-b1bf-a440a01873e5
UUID:
90784e8b-5a54-43ce-b1bf-a440a01873e5
Local pid:
pubs:637450

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