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In vitro synthesis of transfer RNA. I. Purification of required components.

Abstract:

We have described an in vitro system in which active su+III tRNATyr is synthesized from a phi80psu++III DNA template. Using this system, we have identified four essential components that are required for synthesis of tRNA. The first of these is DNA-dependent RNA polymerase. It has been shown that a crude preparation of DNA-dependent RNA polymerase synthesizes su++III tRNATyr precursor similar to that which has been isolated in vivo, and that this preparation is capable of supporting high leve...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Gefter, ML More by this author
Journal:
The Journal of biological chemistry
Volume:
250
Issue:
16
Pages:
6240-6247
Publication date:
1975-08-05
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:901dcdc9-d734-416b-9899-f7c16468ee4e
Source identifiers:
30565
Local pid:
pubs:30565

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