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In vivo and in vitro analysis of the Rhodobacter sphaeroides chemotaxis signaling complexes.

Abstract:

This chapter describes both the in vivo and in vitro methods that have been successfully used to analyze the chemotaxis pathways of R. sphaeroides, showing that two operons each encode a complete chemosensory pathway with each forming into independent signaling clusters. The methods used range from in vitro analysis of the chemotaxis phosphorylation reactions to protein localization experiments. In vitro analysis using purified proteins shows a complex pattern of phosphotransfer. However, pro...

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Publication status:
Published

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Authors


Porter, SL More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Journal:
Methods in enzymology
Volume:
423
Pages:
392-413
Publication date:
2007
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
URN:
uuid:8f91e000-b0da-47bd-aeba-13c5b7689273
Source identifiers:
100296
Local pid:
pubs:100296

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