In vivo and in vitro analysis of the Rhodobacter sphaeroides chemotaxis signaling complexes.
This chapter describes both the in vivo and in vitro methods that have been successfully used to analyze the chemotaxis pathways of R. sphaeroides, showing that two operons each encode a complete chemosensory pathway with each forming into independent signaling clusters. The methods used range from in vitro analysis of the chemotaxis phosphorylation reactions to protein localization experiments. In vitro analysis using purified proteins shows a complex pattern of phosphotransfer. However, pro...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date: