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Two-stage affinity purification for inducibly phosphorylated membrane proteins.

Abstract:

Characterisation of tyrosine phosphorylations induced in immune cells in response to inflammatory stimuli may help elucidate the molecular bases of the diversity of immune responses. We have used anti-phosphotyrosine antibodies in combination with cell surface biotinylation in a two-step affinity purification procedure to recover pervanadate-induced tyrosine phosphorylated proteins from sub-cellular compartments, including the cell surface, of murine T cells and macrophages prior to separatio...

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Publication status:
Published

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Publisher copy:
10.1002/pmic.200401176

Authors


Peirce, MJ More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Proteomics
Volume:
5
Issue:
9
Pages:
2417-2421
Publication date:
2005-06-05
DOI:
EISSN:
1615-9861
ISSN:
1615-9853
URN:
uuid:8f089e2e-01f4-407a-a92d-9a1719b5bc44
Source identifiers:
227462
Local pid:
pubs:227462

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