- Abstract:
-
Characterisation of tyrosine phosphorylations induced in immune cells in response to inflammatory stimuli may help elucidate the molecular bases of the diversity of immune responses. We have used anti-phosphotyrosine antibodies in combination with cell surface biotinylation in a two-step affinity purification procedure to recover pervanadate-induced tyrosine phosphorylated proteins from sub-cellular compartments, including the cell surface, of murine T cells and macrophages prior to separatio...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1002/pmic.200401176
-
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- Journal:
- Proteomics
- Volume:
- 5
- Issue:
- 9
- Pages:
- 2417-2421
- Publication date:
- 2005-06-05
- DOI:
- EISSN:
-
1615-9861
- ISSN:
-
1615-9853
- URN:
-
uuid:8f089e2e-01f4-407a-a92d-9a1719b5bc44
- Source identifiers:
-
227462
- Local pid:
- pubs:227462
- Language:
- English
- Keywords:
- Copyright date:
- 2005
Journal article
Two-stage affinity purification for inducibly phosphorylated membrane proteins.
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