Gas-phase protein assemblies: Unfolding landscapes and preserving native-like structures using noncovalent adducts

Journal article

Mass spectrometry is a rapidly emerging technology for characterising the native structures of protein complexes. One challenge in interpreting results from mass spectrometry experiments is that the structures of protein complexes in the gas phase may differ from those in solution. As such, there is great interest in using small molecules to stabilise the structure of large proteins and their complexes in the gas-phase. Here, we investigate the stabilisation properties of trisH +, a cationic ...

Expand abstract

Authors: Freeke, J; Bush, M; Robinson, C; Ruotolo, B

• Publication status: Published
• Journal: CHEMICAL PHYSICS LETTERS
• Volume: 524
• Pages: 1-9
• Publication date: 2012-02-06
• DOI: 10.1016/j.cplett.2011.11.014
• ISSN: 0009-2614
• Language: English
• Keywords: CCS; CIU; CID; MS; Tris; IM; electrospray ionisation; collision induced dissociation; ESI; collision induced unfolding; TIV; ion mobility; nESI; nano-electrospray ionisation; mass spectrometry; trap injection voltage; TTR; collision cross section; 2-amino-2-hydroxymethyl-propane-1,3-diol; TMV; transition midpoint voltage; tetrameric transthyretin