A naturally occurring variant in human TLR9, P99L, is associated with loss of CpG oligonucleotide responsiveness.

Journal article

The innate immune system employs Toll-like receptors (TLRs) for the detection of invading microorganisms based on distinct molecular patterns. For example, TLR9 is activated by microbial DNA and also by short therapeutic CpG-containing oligonucleotides (CpG-ODN). TLR9 activation leads to the production of interferons and the priming of humoral adaptive immune responses. Unfortunately, the principles of ligand recognition by TLR9 are poorly understood, and genetic variants of TLR9, which may a...

Expand abstract

Authors: Kubarenko, A; Ranjan, S; Rautanen, A; Mills, T; Wong, S

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 285
• Issue: 47
• Pages: 36486-36494
• Publication date: 2010-11-01
• DOI: 10.1074/jbc.M110.117200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Humans; Oligodeoxyribonucleotides; Genotype; Mutagenesis, Site-Directed; Immunoprecipitation; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Cells, Cultured; NF-kappa B; Polymorphism, Single Nucleotide; Mutation; Toll-Like Receptor 9; Kidney; Luciferases; Blotting, Western; Fluorescent Antibody Technique