Independent positioning and action of Escherichia coli replisomes in live cells.

Journal article

A prevalent view of DNA replication has been that it is carried out in fixed "replication factories." By tracking the progression of sister replication forks with respect to genetic loci in live Escherichia coli, we show that at initiation replisomes assemble at replication origins irrespective of where the origins are positioned within the cell. Sister replisomes separate and move to opposite cell halves shortly after initiation, migrating outwards as replication proceeds and both returning ...

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Authors: Reyes-Lamothe, R; Possoz, C; Danilova, O; Sherratt, D

• Publication status: Published
• Journal: Cell
• Volume: 133
• Issue: 1
• Pages: 90-102
• Publication date: 2008-04-01
• DOI: 10.1016/j.cell.2008.01.044
• EISSN: 1097-4172
• ISSN: 0092-8674
• Language: English
• Keywords: DNA Replication; Escherichia coli Proteins; DNA Replication Timing; Chromosomes, Bacterial; Replication Origin; DNA, Bacterial; Replicon; Escherichia coli