Abundant non-inclusion α-synuclein pathology in Lewy body-negative LRRK2-mutant cases

Journal article

Lewy body diseases are common neurodegenerative diseases, including Parkinson’s disease (PD) and dementia with Lewy bodies, which lead to both motor and non-motor symptoms. They are neuropathologically characterized by loss of neuromelanized neurons in the substantia nigra pars compacta and α-synuclein-immunopositive inclusions (Lewy bodies) in several types of neurons in the brain. A fraction of monogenic PD cases, however, represent a conundrum, as they can present with clinical Lewy body disease but do not have Lewy bodies upon neuropathological examination. For LRRK2, the presence or absence of Lewy bodies is not related to any specific mutation in the gene and different clinical presentation and neuropathology can be present even in the same family. Here, we present the first evidence of widespread α-synuclein accumulation detected with proximity ligation assay (PLA) using the MJFR14-6-4-2 antibody in six Lewy body-negative LRRK2 cases and compare the levels with five patients with neuropathologically verified Lewy body disease and six healthy controls. We show that non-inclusion aggregated α-synuclein in the form of particulate PLA signal is dominant in the LRRK2 cases, while both Lewy-like and particulate PLA signal is found in late-stage Lewy body disease. Furthermore, LRRK2 cases displayed prominent particulate PLA signal in pontocerebellar tracts and inferior olivary nuclei in the brainstem, which was not seen in idiopathic Lewy body disease cases. These results suggest that Lewy-body negative LRRK2-related PD is not associated with a lack of α-synuclein aggregation in neurons but rather a deficiency in the formation of inclusions.

Authors: Jensen, NM; Vitic, Z; Antorini, MR; Viftrup, TB; Parkkinen, L

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Springer
• Journal: Acta Neuropathologica
• Volume: 149
• Issue: 1
• Article number: 41
• Publication date: 2025-05-02
• Acceptance date: 2025-03-21
• DOI: 10.1007/s00401-025-02871-w
• EISSN: 1432-0533
• ISSN: 0001-6322
• Language: English
• Keywords: Proximity ligation assay; LRRK2; Non-inclusion pathology; α-Synuclein; Lewy body disease; Neurodegeneration