Development of a solid-phase assay for analysis of matrix metalloproteinase activity.

Journal article

Proteases play fundamentally important roles in normal physiology and disease pathology. Methods for detection of active proteolysis may greatly aid in the diagnosis of disease progression, and suggest modes of therapeutic intervention. Most assays for proteolytic potential are limited by a lack of specificity and/or quantification. We have developed a solid-phase activity assay for members of the matrix metalloproteinase (MMP) family that is specific and can be used to quantify active enzyme...

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Authors: Lauer-Fields, J; Nagase, H; Fields, G

• Journal: Journal of biomolecular techniques : JBT
• Volume: 15
• Issue: 4
• Pages: 305-316
• Publication date: 2004-12-01
• EISSN: 1943-4731
• ISSN: 1524-0215
• Language: English
• Keywords: Amino Acid Sequence; Humans; Molecular Sequence Data; Substrate Specificity; Enzyme-Linked Immunosorbent Assay; Melanoma; Matrix Metalloproteinases