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Conference item : Abstract

Detrending: how to correct images for bleaching

Abstract:
Image series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence fluctuation spectroscopy. We outline mistakes that are often made when detrending. We provide a free, user-friendly software for correctly detrending images.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2017.11.1926

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Publisher:
Cell Press
Host title:
Biophysical Journal
Journal:
Biophysical Journal More from this journal
Volume:
114
Issue:
3
Pages:
345A-345A
Publication date:
2018-02-06
DOI:
ISSN:
0006-3495
Subtype:
Abstract
Pubs id:
pubs:853458
UUID:
uuid:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fd
Local pid:
pubs:853458
Source identifiers:
853458
Deposit date:
2018-05-23

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