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Detrending: how to correct images for bleaching

Subtitle:
Conference abstract
Abstract:
Image series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence fluctuation spectroscopy. We outline mistakes that are often made when detrending. We provide a free, user-friendly software for correctly detrending images.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2017.11.1926

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
Publisher:
Cell Press Publisher's website
Volume:
114
Issue:
3
Pages:
345A-345A
Publication date:
2018-02-06
DOI:
ISSN:
0006-3495
Pubs id:
pubs:853458
URN:
uri:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fd
UUID:
uuid:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fd
Local pid:
pubs:853458

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