How Forster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology.

Journal article

Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (no...

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Authors: Fruhwirth, G; Fernandes, L; Weitsman, G; Patel, G; Kelleher, M

• Publication status: Published
• Journal: Chemphyschem : a European journal of chemical physics and physical chemistry
• Volume: 12
• Issue: 3
• Pages: 442-461
• Publication date: 2011-02-01
• DOI: 10.1002/cphc.201000866
• EISSN: 1439-7641
• ISSN: 1439-4235
• Language: English
• Keywords: Fluorescence Resonance Energy Transfer; Humans; Fluorescent Dyes; Neoplasms; Computational Biology; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Proteins