Journal article
A comparison of protein kinases inhibitor screening methods using both enzymatic activity and binding affinity determination
- Abstract:
- Binding assays are increasingly used as a screening method for protein kinase inhibitors; however, as yet only a weak correlation with enzymatic activity-based assays has been demonstrated. We show that the correlation between the two types of assays can be improved using more precise screening conditions. Furthermore a marked improvement in the correlation was found by using kinase constructs containing the catalytic domain in presence of additional domains or subunits.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Version of record, pdf, 294.5KB)
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- Publisher copy:
- 10.1371/journal.pone.0098800
Authors
Bibliographic Details
- Publisher:
- Public Library of Science Publisher's website
- Journal:
- PLoS ONE Journal website
- Volume:
- 9
- Issue:
- 6
- Pages:
- e98800
- Publication date:
- 2014-06-10
- Acceptance date:
- 2014-04-27
- DOI:
- EISSN:
-
1932-6203
- ISSN:
-
1932-6203
Item Description
- Language:
- English
- Keywords:
- UUID:
-
uuid:8d55f8b2-d046-4af0-a79c-5c4b4a6fd60e
- Local pid:
- pubs:469645
- Source identifiers:
-
469645
- Deposit date:
- 2014-06-30
Terms of use
- Copyright holder:
- Rudolf et al
- Copyright date:
- 2014
- Notes:
- © 2014 Rudolf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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