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Journal article

Expression and characterisation of recombinant oligomeric envelope glycoproteins derived from primary isolates of HIV-1.

Abstract:

The production, purification and characterisation of recombinant gp140 oligomeric envelope glycoproteins derived from six primary isolates of HIV-1 (covering clades A, B, C, D, F and O) are described. Using a Chinese hamster ovary cell expression system, expression levels of between 0.1 and 1 mg/l cell-conditioned culture media were obtained, and purified to >95% by affinity chromatography. A, B, D, F and O clade gp 140s were found to be multimeric, bind to a panel of defined env-specific ...

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Publication status:
Published

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Journal:
Vaccine
Volume:
22
Issue:
8
Pages:
1032-1046
Publication date:
2004-02-05
DOI:
EISSN:
1873-2518
ISSN:
0264-410X
URN:
uuid:8d559e43-ff69-4f30-bc2c-7f426a6f8125
Source identifiers:
18884
Local pid:
pubs:18884

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