- Abstract:
-
The production, purification and characterisation of recombinant gp140 oligomeric envelope glycoproteins derived from six primary isolates of HIV-1 (covering clades A, B, C, D, F and O) are described. Using a Chinese hamster ovary cell expression system, expression levels of between 0.1 and 1 mg/l cell-conditioned culture media were obtained, and purified to >95% by affinity chromatography. A, B, D, F and O clade gp 140s were found to be multimeric, bind to a panel of defined env-specific ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.vaccine.2003.08.042
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- Journal:
- Vaccine
- Volume:
- 22
- Issue:
- 8
- Pages:
- 1032-1046
- Publication date:
- 2004-02-05
- DOI:
- EISSN:
-
1873-2518
- ISSN:
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0264-410X
- URN:
-
uuid:8d559e43-ff69-4f30-bc2c-7f426a6f8125
- Source identifiers:
-
18884
- Local pid:
- pubs:18884
- Copyright date:
- 2004
Journal article
Expression and characterisation of recombinant oligomeric envelope glycoproteins derived from primary isolates of HIV-1.
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