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3D correlative cryo-structured illumination fluorescence and soft x-ray microscopy elucidates reovirus intracellular release pathway

Abstract:

Imaging of biological matter across resolution scales entails the challenge of preserving the direct and unambiguous correlation of subject features from the macroscopic to the microscopic level. Here, we present a correlative imaging platform developed specifically for imaging cells in 3D under cryogenic conditions by using X-rays and visible light. Rapid cryo-preservation of biological specimens is the current gold standard in sample preparation for ultrastructural analysis in X-ray imaging...

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Publication status:
Published
Peer review status:
Peer reviewed

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Division:
UAS
Subgroup:
Undergraduate Admissions and Outreach
Oxford college:
New College
Role:
Author
ORCID:
0000-0001-9877-0457
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Role:
Author
ORCID:
0000-0002-4822-165X
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Publisher:
Elsevier Publisher's website
Journal:
Cell Journal website
Volume:
182
Issue:
2
Pages:
515-530.e17
Publication date:
2020-07-01
Acceptance date:
2020-05-28
DOI:
EISSN:
1097-4172
ISSN:
0092-8674
Pmid:
32610083
Pubs id:
1115904
Local pid:
pubs:1115904
Language:
English
Keywords:
Format:
Print-Electronic

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