Journal article
Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues.
- Abstract:
- The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked.
- Publication status:
- Published
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- Publisher copy:
- 10.1016/0014-5793(92)81147-e
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Authors
Bibliographic Details
- Journal:
- FEBS letters
- Volume:
- 310
- Issue:
- 1
- Pages:
- 63-65
- Publication date:
- 1992-09-01
- DOI:
- EISSN:
-
1873-3468
- ISSN:
-
0014-5793
- Source identifiers:
-
59798
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:59798
- UUID:
-
uuid:8d4863cf-5d83-44ab-b4f0-a1fe70429c9f
- Local pid:
- pubs:59798
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 1992
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