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The effect of solution pH on the structure of lysozyme layers adsorbed at the silica-water interface studied by neutron reflection

Abstract:

We have studied the structure of lysozyme layers adsorbed at the silica-water interface using specular neutron reflection. The effect of pH on the adsorbed lysozyme layer was examined by manipulating the pH in two different cycles at two constant lysozyme concentrations of 0.03 and 1.0 g dm-3; the first cycle was started at pH = 4 followed by pH = 7 and then 8, before returning to 4; the second cycle was started at pH = 7 followed by a decrease to 4 and then back to 7. The neutron reflectivit...

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Publication status:
Published

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Publisher copy:
10.1021/la970623z

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
LANGMUIR More from this journal
Volume:
14
Issue:
2
Pages:
438-445
Publication date:
1998-01-20
DOI:
EISSN:
1520-5827
ISSN:
0743-7463
Language:
English
Pubs id:
pubs:36324
UUID:
uuid:8c111d18-7095-448f-b24a-95ac54544f43
Local pid:
pubs:36324
Source identifiers:
36324
Deposit date:
2012-12-19

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