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Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.

Abstract:
Yeast artificial chromosome (YAC) libraries have been constructed from a variety of organisms using different approaches. This protocol outlines in detail the construction of YAC libraries with large inserts using size fractionation of partially digested DNA by pulsed-field gel electrophoresis.

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Publisher copy:
10.1007/bf02921692

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Role:
Author
Publisher:
Humana Press
Journal:
Molecular biotechnology
Volume:
1
Issue:
3
Pages:
241-249
Publication date:
1994-06-05
DOI:
EISSN:
1559-0305
ISSN:
1073-6085
URN:
uuid:8c056b77-9f9d-4830-b44a-7aea376bc173
Source identifiers:
35720
Local pid:
pubs:35720

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