- Abstract:
- Yeast artificial chromosome (YAC) libraries have been constructed from a variety of organisms using different approaches. This protocol outlines in detail the construction of YAC libraries with large inserts using size fractionation of partially digested DNA by pulsed-field gel electrophoresis.
- Publisher copy:
- 10.1007/bf02921692
-
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- Publisher:
- Humana Press
- Journal:
- Molecular biotechnology
- Volume:
- 1
- Issue:
- 3
- Pages:
- 241-249
- Publication date:
- 1994-06-05
- DOI:
- EISSN:
-
1559-0305
- ISSN:
-
1073-6085
- URN:
-
uuid:8c056b77-9f9d-4830-b44a-7aea376bc173
- Source identifiers:
-
35720
- Local pid:
- pubs:35720
- Copyright date:
- 1994
Journal article
Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
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