Journal article icon

Journal article

Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes.

Abstract:
The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.
Publication status:
Published

Actions


Access Document


Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Journal:
AIDS (London, England) More from this journal
Volume:
18
Issue:
11
Pages:
1595-1598
Publication date:
2004-07-01
DOI:
EISSN:
1473-5571
ISSN:
0269-9370
Language:
English
Keywords:
Pubs id:
pubs:95273
UUID:
uuid:8bf0917e-fec8-4e2c-8228-4cfc7049696d
Local pid:
pubs:95273
Source identifiers:
95273
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP