- Abstract:
-
We used mass spectrometry to identify proteins that are released in the gas phase from Escherichia coli ribosomes in response to a range of different solution conditions and cofactor binding. From solution at neutral pH the spectra are dominated by just 4 of the 54 ribosomal proteins (L7/L12, L11, and L10). Lowering the pH of the solution leads to the gas phase dissociation of four additional proteins as well as the 5 S RNA. Replacement of Mg(2+) by Li(+) ions in solutions of ribosomes induce...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1074/jbc.m208966200
-
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- Journal:
- The Journal of biological chemistry
- Volume:
- 278
- Issue:
- 2
- Pages:
- 1259-1267
- Publication date:
- 2003-01-05
- DOI:
- EISSN:
-
1083-351X
- ISSN:
-
0021-9258
- URN:
-
uuid:8bca6c87-b7cc-4ad0-9acb-8c22f43543c7
- Source identifiers:
-
59245
- Local pid:
- pubs:59245
- Copyright date:
- 2003
Journal article
Dissociation of intact Escherichia coli ribosomes in a mass spectrometer. Evidence for conformational change in a ribosome elongation factor G complex.
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