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Dissociation of intact Escherichia coli ribosomes in a mass spectrometer. Evidence for conformational change in a ribosome elongation factor G complex.

Abstract:

We used mass spectrometry to identify proteins that are released in the gas phase from Escherichia coli ribosomes in response to a range of different solution conditions and cofactor binding. From solution at neutral pH the spectra are dominated by just 4 of the 54 ribosomal proteins (L7/L12, L11, and L10). Lowering the pH of the solution leads to the gas phase dissociation of four additional proteins as well as the 5 S RNA. Replacement of Mg(2+) by Li(+) ions in solutions of ribosomes induce...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m208966200

Authors


Hanson, CL More by this author
Nierhaus, KH More by this author
Robinson, CV More by this author
Journal:
The Journal of biological chemistry
Volume:
278
Issue:
2
Pages:
1259-1267
Publication date:
2003-01-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:8bca6c87-b7cc-4ad0-9acb-8c22f43543c7
Source identifiers:
59245
Local pid:
pubs:59245

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