Journal article icon

Journal article

A time- and cost-efficient system for high-level protein production in mammalian cells.

Abstract:

Most proteins for structural biology studies are produced by high-level expression in Escherichia coli. However, prokaryotic based expression systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based expression systems have been developed. Of these, yeast and baculovirus-infected insect cells currently represent the expression systems of choice for structural biologists. Here, protocols for a simple, fast and affordable method for tran...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1107/s0907444906029799

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Acta crystallographica. Section D, Biological crystallography More from this journal
Volume:
62
Issue:
Pt 10
Pages:
1243-1250
Publication date:
2006-10-01
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Language:
English
Keywords:
Pubs id:
pubs:31320
UUID:
uuid:8b9b13e0-0800-43e7-b935-84d5410086a9
Local pid:
pubs:31320
Source identifiers:
31320
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP