Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture.
Single-domain intracellular antibodies are antibody variable segments that bind to specific target proteins inside cells. These antigen-binding variable regions can interfere with protein function or perturb protein-protein interactions and can be used as tools for research, especially functional genomics and proteomics and interfering with the protein interactome. This protocol (Intracellular Antibody Capture, IAC(3)) describes the isolation of functional variable heavy (VH) or variable ligh...Expand abstract
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