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Journal article

Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture.

Abstract:

Single-domain intracellular antibodies are antibody variable segments that bind to specific target proteins inside cells. These antigen-binding variable regions can interfere with protein function or perturb protein-protein interactions and can be used as tools for research, especially functional genomics and proteomics and interfering with the protein interactome. This protocol (Intracellular Antibody Capture, IAC(3)) describes the isolation of functional variable heavy (VH) or variable ligh...

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Publisher copy:
10.1038/nprot.2009.199

Authors


Journal:
Nature protocols
Volume:
5
Issue:
1
Pages:
67-92
Publication date:
2010-01-01
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Source identifiers:
324311
Language:
English
Keywords:
Pubs id:
pubs:324311
UUID:
uuid:8b878cda-ddf3-4a49-84ef-63c309445f46
Local pid:
pubs:324311
Deposit date:
2013-11-16

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