Vacuolar chloride transport in Mesembryanthemum crystallinum L. measured using the fluorescent dye lucigenin.

Journal article

To study vacuolar chloride (Cl(-)) transport in the halophilic plant Mesembryanthemum crystallinum L., Cl(-) uptake into isolated tonoplast vesicles was measured using the Cl(-)-sensitive fluorescent dye lucigenin (N,N'-dimethyl-9,9'-bisacridinium dinitrate). Lucigenin was used at excitation and emission wavelengths of 433 nm and 506 nm, respectively, and showed a high sensitivity towards Cl(-), with a Stern-Volmer constant of 173 m(-1) in standard assay buffer. While lucigenin fluorescence w...

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Authors: Wissing, F; Smith, J

• Publication status: Published
• Journal: Journal of membrane biology
• Volume: 177
• Issue: 3
• Pages: 199-208
• Publication date: 2000-10-01
• DOI: 10.1007/s002320010003
• EISSN: 1432-1424
• ISSN: 0022-2631
• Language: English
• Keywords: Acridines; Ion Transport; Chlorides; Dimethyl Sulfoxide; Fluorescent Dyes; Angiosperms; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Vacuoles; Kinetics; Flufenamic Acid; Hydrogen-Ion Concentration