Activation and inactivation of the osteoclast Ca2+ receptor by the trivalent cation, La3+.

Journal article

We report changes in the cytosolic Ca2+ concentration ([Ca2+]i) of single rat osteoclasts in response to Ca2+ receptor activation by micromolar concentrations of the lanthanide metal cation, La3+. The extracellular application of La3+ induced a concentration-dependent elevation of cytosolic [Ca2+]. Prior conditioning of osteoclasts with La3+ resulted in a concentration-dependent reduction of the response to a subsequent application of a maximally effective concentration of Ni2+, a known agonist of the osteoclast Ca2+ receptor. The results establish that the osteoclast Ca2+ receptor is highly sensitive to activation and inactivation by the trivalent cation, La3+.

Authors: Shankar, V; Alam, A; Bax, C; Bax, B; Pazianas, M

• Journal: Biochemical and biophysical research communications
• Volume: 187
• Issue: 2
• Pages: 907-912
• Publication date: 1992-09-01
• DOI: 10.1016/0006-291x(92)91283-v
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Lanthanum; Rats; Animals, Newborn; Calcium; Rats, Inbred Strains; Cytosol; Calcium-Binding Proteins; Spectrometry, Fluorescence; Nickel; Animals; Osteoclasts