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Butane and propane oxidation by engineered cytochrome P450cam.

Abstract:
The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidizes butane with a turnover rate of 750 min-1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min-1 with 4% yield.
Publication status:
Published

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Publisher copy:
10.1039/b110957j

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Journal:
Chemical Communications More from this journal
Issue:
5
Pages:
490-491
Publication date:
2002-03-01
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Language:
English
Keywords:
Pubs id:
pubs:32156
UUID:
uuid:8b2dc260-e559-4730-ae1c-c83d757cdde3
Local pid:
pubs:32156
Source identifiers:
32156
Deposit date:
2012-12-19

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