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Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.

Abstract:
Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries.

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Publisher copy:
10.1385/0-89603-229-9:225

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Lehrach, H More by this author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
12
Pages:
225-234
Publication date:
1992
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:8b187b76-4a20-4a53-ad23-b45575911e32
Source identifiers:
127150
Local pid:
pubs:127150
Language:
English

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