Journal article
Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
- Abstract:
- Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries.
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Bibliographic Details
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Volume:
- 12
- Pages:
- 225-234
- Publication date:
- 1992-01-01
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Source identifiers:
-
127150
Item Description
- Language:
- English
- Pubs id:
-
pubs:127150
- UUID:
-
uuid:8b187b76-4a20-4a53-ad23-b45575911e32
- Local pid:
- pubs:127150
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 1992
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