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Streptavidin-polyvinylamine conjugates labeled with a europium chelate: applications in immunoassay, immunohistochemistry, and microarrays.

Abstract:

BACKGROUND: The favorable properties of lanthanide chelates compared with conventional fluorescent probes have attracted considerable interest. A Eu(3+) chelator, 4,7-bis(chlorosulfophenyl)-1, 10-phenanthroline-2,9-dicarboxylic acid (BCPDA), has been synthesized previously. METHODS: We here describe immunoassay, immunohistochemistry, and microarray applications of a new streptavidin-based universal polyvinylamine (PVA) detection reagent that is multiply labeled with the europium chelate of BC...

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Authors


Scorilas, A More by this author
Bjartell, A More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Surgical Sciences
Diamandis, EP More by this author
Journal:
Clinical chemistry
Volume:
46
Issue:
9
Pages:
1450-1455
Publication date:
2000-09-05
EISSN:
1530-8561
ISSN:
0009-9147
URN:
uuid:8b0285a0-fae7-4f8b-898f-d950fe5e4677
Source identifiers:
366051
Local pid:
pubs:366051

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