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A rapid, quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro.

Abstract:

Alkaline phosphatase (ALP) is the most widely recognized biochemical marker for osteoblast activity. Although its precise function is poorly understood, it is believed to play a role in skeletal mineralization. The aim of this study was to develop an assay suitable for measuring the activity of this enzyme in microtiter plate format. Using the well-characterized osteoblast-like cell line Saos-2, this paper describes an optimized biochemical assay suitable for measuring ALP activity in tissue ...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Role:
Author
Journal:
Bone and mineral
Volume:
27
Issue:
1
Pages:
57-67
Publication date:
1994-10-05
DOI:
ISSN:
0169-6009
URN:
uuid:8b00eb28-d886-4ed9-97bd-a4c7036dc4b1
Source identifiers:
103915
Local pid:
pubs:103915

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