- Abstract:
-
Alkaline phosphatase (ALP) is the most widely recognized biochemical marker for osteoblast activity. Although its precise function is poorly understood, it is believed to play a role in skeletal mineralization. The aim of this study was to develop an assay suitable for measuring the activity of this enzyme in microtiter plate format. Using the well-characterized osteoblast-like cell line Saos-2, this paper describes an optimized biochemical assay suitable for measuring ALP activity in tissue ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/S0169-6009(08)80187-0
-
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- Journal:
- Bone and mineral
- Volume:
- 27
- Issue:
- 1
- Pages:
- 57-67
- Publication date:
- 1994-10-05
- DOI:
- ISSN:
-
0169-6009
- URN:
-
uuid:8b00eb28-d886-4ed9-97bd-a4c7036dc4b1
- Source identifiers:
-
103915
- Local pid:
- pubs:103915
- Copyright date:
- 1994
Journal article
A rapid, quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro.
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