Alkaline phosphatase (ALP) is the most widely recognized biochemical marker for osteoblast activity. Although its precise function is poorly understood, it is believed to play a role in skeletal mineralization. The aim of this study was to develop an assay suitable for measuring the activity of this enzyme in microtiter plate format. Using the well-characterized osteoblast-like cell line Saos-2, this paper describes an optimized biochemical assay suitable for measuring ALP activity in tissue ...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
A rapid, quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record