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Journal article

Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy.

Abstract:

To be able to detect in situ changes in protein conformation without perturbing the physiological environment would be a major step forward in understanding the precise mechanism occurring in protein interaction. We have developed a novel approach to monitoring conformational changes of proteins in intact cells. A double-labelled fluorescent green fluorescent protein-yellow fluorescent protein (GFP-YFP) fusion protein has been constructed, allowing the exploitation of enhanced-acceptor-fluore...

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Publication status:
Published

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Publisher copy:
10.1042/bj20030358

Authors


Calleja, V More by this author
Ameer-Beg, SM More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Oncology
Woscholski, R More by this author
Downward, J More by this author
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Journal:
The Biochemical journal
Volume:
372
Issue:
Pt 1
Pages:
33-40
Publication date:
2003-05-05
DOI:
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:8a98179a-8632-46f7-8c0c-d6ecb2118a63
Source identifiers:
131359
Local pid:
pubs:131359

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