Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation.

Journal article

For more than a decade Liberase HI was commonly used as the standard enzyme blend for clinical human islet isolation until enforced replacement by collagenase NB1 (NB1). This change resulted initially in a reduction in islet isolation outcome and transplant activities worldwide. This retrospective study was initiated to compare the efficiency of NB1 premium grade with Liberase in 197 human islet isolations. All pancreata were processed between January 2006 and June 2008 utilizing the same pro...

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Authors: Brandhorst, H; Friberg, A; Friberg, A; Nilsson, B; Nilsson, B

• Journal: Cell transplantation
• Volume: 19
• Issue: 1
• Pages: 3-8
• Publication date: 2010-01-01
• DOI: 10.3727/096368909x477507
• EISSN: 1555-3892
• ISSN: 0963-6897
• Language: English
• Keywords: Humans; Cell Survival; Aged; Collagenases; Islets of Langerhans Transplantation; Tissue and Organ Harvesting; Adolescent; Male; Cells, Cultured; Adult; Young Adult; Islets of Langerhans; Cell Separation; Female; Thermolysin; Cell Culture Techniques; Middle Aged; Retrospective Studies; Graft Survival