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In vivo gene transfer by electroporation allows expression of a fluorescent transgene in hamster testis and epididymal sperm and has no adverse effects upon testicular integrity or sperm quality.

Abstract:

The study of gene function in testis and sperm has been greatly assisted by transgenic mouse models. Recently, an alternative way of expressing transgenes in mouse testis has been developed that uses electroporation to introduce transgenes into the male germ cells. This approach has been successfully used to transiently express reporter genes driven by constitutive and testis-specific promoters. It has been proposed as an alternative method for studying gene function in testis and sperm, and ...

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Publication status:
Published

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Journal:
Biology of reproduction
Volume:
74
Issue:
1
Pages:
95-101
Publication date:
2006-01-05
DOI:
EISSN:
1529-7268
ISSN:
0006-3363
URN:
uuid:8a7ecc40-1bd5-416a-be9e-2750ab95cd68
Source identifiers:
185302
Local pid:
pubs:185302

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